The ExoVista Exosome isolation Kit is optimized for isolation of exosomes from cell culture media. The kit includes precipitation reagent which is used to isolate exosomes and stabilizer solution contained phosphate buffer saline for exosome resuspension.
Exosomes are generally believed to be 20 – 120 nanometer particles which are excreted from cells . These particles approximately are the same size as viruses but considerably smaller than microvesicles. Exosomes are excreted from cells into the surrounding media and can be found in various body fluids. Their role as intercellular messengers in addition to their stability as carrier of proteins and RNA, make them potential biomarkers for a variety of diseases, biological processes and potential treatment.
ExoVista Kit Specifications
Exosomes isolated from cell culture media by ExoVista kit can be detected and be confirmed as exosomes by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and IHC by CD63 and CD81 biomarkers.